9WYG

Crystal structure of HAstV8 spike

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 2
Unit cell lengths	66.757, 53.709, 56.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.380 - 1.440
R-factor	0.166
Rwork	0.164
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.725
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.440	1.440
Rpim		0.030
Number of reflections	443248	37035
<I/σ(I)>	14.88
Completeness [%]	99.8
Redundancy	11.96
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	0.1 M HEPES pH 7.5, 30% w/v Polyethylene glycol 1,000