9WYE

Crystal structure of HAstV1 spike

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978530
Spacegroup name	P 41 21 2
Unit cell lengths	103.624, 103.624, 41.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.960 - 1.350
R-factor	0.1491
Rwork	0.148
R-free	0.17660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.350	1.350
Rmerge	0.072
Rpim		0.015
Number of reflections	1095258	49000
<I/σ(I)>	26.01
Completeness [%]	98.1
Redundancy	22.35
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	0.2 M Lithium sulfate, 0.1 M Sodium acetate, PH 4.5, 50% w/v PEG 400