9WYC
Crystal structure of the Aspergillus HVM2 protein in complex with AKG, nickel, and the substrate chrysosporazine D
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-27 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 62 |
| Unit cell lengths | 100.931, 100.931, 97.324 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.700 - 1.950 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0rc1.5555) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 1.950 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 40998 | 40998 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 93.7 | |
| Redundancy | 3.59 | |
| CC(1/2) | 0.750 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.2M magnesium chloride, 0.1M Tris pH 8.0, 20% W/V PEG 3350 |
