9WX3

Structure of flagellar hook subunit FlgE D-II domain in Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	STFC Large Pixel Detector
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	55.453, 75.304, 89.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.175 - 1.800
R-factor	0.1681
Rwork	0.166
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	35970	3512
<I/σ(I)>	18.08
Completeness [%]	100.0
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Sodium HEPES (pH 7.0) and 15% (w/v) PEG 20,000