9WW1
Oxy-bound rHb0.1WT beta homotetramer human hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-12 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.804, 80.947, 62.683 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 22.497 - 2.000 |
| Rwork | 0.195 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1abw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.855 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.457 | |
| Number of reflections | 36366 | 2650 |
| <I/σ(I)> | 16.1 | 1.6 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.02 | 2.76 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.8 | 297.15 | 1.2 M Tri-sodium citrate pH 8.8, 15% PEG 8000 |
