9WVH
Crystal structure of TONSL UBL domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS DIAMOND MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-19 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 112.147, 112.147, 69.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.900 - 1.960 |
| R-factor | 0.19461 |
| Rwork | 0.194 |
| R-free | 0.20884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.900 | 2.080 |
| High resolution limit [Å] | 1.956 | 1.960 |
| Rmerge | 0.117 | 1.769 |
| Rmeas | 0.121 | |
| Rpim | 0.031 | 0.484 |
| Number of reflections | 15431 | 5068 |
| <I/σ(I)> | 18.07 | 1.61 |
| Completeness [%] | 99.9 | 99.92 |
| Redundancy | 15 | 14.2 |
| CC(1/2) | 0.999 | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris-bicine pH 8.5, 0.5% PEG monomethyl ether 5000, 0.8 M Potassium sodium tartrate tetrahydrate, 10 mM DTT |
