9WV8
Crystal structure of phosphate binding protein(PstS1) from Synechocystis sp. PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.718, 64.816, 129.058 |
| Unit cell angles | 90.00, 91.27, 90.00 |
Refinement procedure
| Resolution | 45.720 - 1.900 |
| R-factor | 0.1784 |
| Rwork | 0.176 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.906 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.720 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 49129 | 4805 |
| <I/σ(I)> | 12.21 | |
| Completeness [%] | 99.3 | |
| Redundancy | 1 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 0.2M MgCl2, 0.1M Tris pH 9.0, 30% PEG 3000 |
