9WV4

Crystal structure of human ZMYND8 MYND domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-18
Detector	Bruker PHOTON III
Wavelength(s)	1.5406
Spacegroup name	P 21 21 21
Unit cell lengths	63.705, 71.188, 230.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.670 - 2.290
R-factor	0.2441
Rwork	0.242
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.942
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.670	2.340
High resolution limit [Å]	2.290	2.290
Rmerge	0.126	1.210
Rmeas	0.135
Rpim	0.049	0.522
Number of reflections	47990	5091
<I/σ(I)>	12.16	1.25
Completeness [%]	99.6	95.65
Redundancy	7.4	6
CC(1/2)	0.998	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.75 M ammonium sulfate, 2% PEG-3350, 0.1 M MES, pH 6.5