9WSC
Crystal structure of PcNLP485 effector protein from P.capsici
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.764, 63.366, 79.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.759 - 1.698 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.20380 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.063 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.759 |
| High resolution limit [Å] | 1.698 | 1.698 |
| Rmeas | 0.065 | 0.337 |
| Number of reflections | 27657 | 2722 |
| <I/σ(I)> | 27.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1M BIS-TRIS propane pH 7.2, 3.6 M sodium nitrate |
