9WRN
Crystal structure of chimeric anti-Z-DNA Fab cZ22-Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 07A |
| Synchrotron site | NSRRC |
| Beamline | TPS 07A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2024-01-02 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.016, 87.025, 149.400 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 18.020 - 2.150 |
| R-factor | 0.1945 |
| Rwork | 0.192 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.020 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 101636 | 6578 |
| <I/σ(I)> | 12.58 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.43 | |
| CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.16 M ammonium sulfate, 20% (w/v) polyethylene glycol (PEG) 4,000, 20% (v/v) glycerol, and 0.08 M sodium acetate trihydrate, pH 4.6 |
