9WQU

b-b' domain fragment of ER-60 (ERp57) under microgravity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-07
Detector	RAYONIX MX225HE
Wavelength(s)	0.75
Spacegroup name	P 1 21 1
Unit cell lengths	74.315, 62.901, 97.148
Unit cell angles	90.00, 98.64, 90.00

Refinement procedure

Resolution	47.780 - 1.800
R-factor	0.22081
Rwork	0.219
R-free	0.26272
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h8l
RMSD bond length	0.009
RMSD bond angle	2.035
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	47.780	1.910
High resolution limit [Å]	1.800	5.370	1.800
Rmerge	0.029	0.014	0.297
Rmeas	0.041	0.019	0.420
Number of reflections	82113	2217	9293
<I/σ(I)>	9.31
Completeness [%]	99.4
Redundancy	3.84
CC(1/2)	0.999	0.999	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION	7	293	50 mM HEPES-NaOH, pH 7.0, 20% PEG3350, 50 mM Ammonium Sulfate, 100 m M NaCl, 0.04% NaN3