9WQ1

Crystal structure of CNOT6L and YL-333P complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97923
Spacegroup name	P 32 2 1
Unit cell lengths	78.737, 78.737, 171.726
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.840 - 2.090
R-factor	0.2763
Rwork	0.274
R-free	0.31860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.283
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.090	2.090
Number of reflections	37580	2717
<I/σ(I)>	6.44
Completeness [%]	100.0	100
Redundancy	18.79
CC(1/2)	0.975	0.016

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M Hepes, pH 7.5, 1.1M ammonium tartrate, 0.2M NDSB-201