9WPK

structure of Mycoplasma hyopneumoniae Leucine aminopeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9789
Spacegroup name	I 41 2 2
Unit cell lengths	218.715, 218.715, 301.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.780 - 2.150
R-factor	0.1906
Rwork	0.190
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.580
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.150	2.150
Rmerge	0.106	3.214
Rpim	0.021	0.767
Number of reflections	195882	31394
<I/σ(I)>	22.65	1.11
Completeness [%]	100.0
Redundancy	26.8
CC(1/2)	0.999	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Sodium acetate trihydrate and 2.0 M Sodium formate at pH 4.6