9WPA
Complex structure of anti-CRISPR-associated protein Aca7 and promoter DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 125 |
| Detector technology | PIXEL |
| Collection date | 2025-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.430, 113.650, 87.770 |
| Unit cell angles | 90.00, 103.84, 90.00 |
Refinement procedure
| Resolution | 29.200 - 2.940 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 3.045 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Number of reflections | 16286 | 1717 |
| <I/σ(I)> | 7.18 | |
| Completeness [%] | 94.3 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.977 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20%(w/v) PEG 1000 0.1M Tris-base/HCl pH7.0 |
