9WOY

Crystal Structure of the MLH1 Protein Bound to the FAN1 Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	43.018, 60.151, 250.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.080 - 2.280
Rwork	0.241
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.460	2.240
High resolution limit [Å]	2.180	2.180
Number of reflections	33445	2233
<I/σ(I)>	5.9
Completeness [%]	98.1
Redundancy	11.9
CC(1/2)	0.961	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.2 M Trimethylamine N-oxide dihydrate, 0.1 M Tris pH 8.5, 20% w/v Polyethylene glycol monomethyl ether 2,000