9WOO

VcCdnG,a CD-NTase from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	108.836, 118.112, 78.071
Unit cell angles	90.00, 119.49, 90.00

Refinement procedure

Resolution	19.860 - 2.470
R-factor	0.1981
Rwork	0.197
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.418
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1-4487-000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.560
High resolution limit [Å]	2.470	2.470
Rmerge	0.058	0.604
Rmeas	0.068	0.722
Rpim	0.036	0.391
Number of reflections	30871	6007
<I/σ(I)>	14.3	1.9
Completeness [%]	99.8	99.9
Redundancy	3.4
CC(1/2)	0.998	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	HEPES,sodium chloride