9WNP
Ambient Apo SIRT2 structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2025-06-12 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.520, 74.857, 55.532 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 29.250 - 2.700 |
| R-factor | 0.2244 |
| Rwork | 0.224 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8xe7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.780 |
| Data reduction software | CrysalisPro (1.171.42.35a) |
| Data scaling software | CrysalisPro (1.171.42.35a) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.990 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rpim | 0.158 | 0.730 |
| Number of reflections | 7751 | 396 |
| <I/σ(I)> | 5.57 | 0.27 |
| Completeness [%] | 93.5 | 70.5 |
| Redundancy | 9.9 | 2 |
| CC(1/2) | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 298 | 23% (w/v) 3350 peg 0.1 M Bis-Tris pH 6 |
