9WM3
Crystal structure of the apo form of Escherichia coli RecG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-05-20 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 147.937, 147.937, 178.251 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 128.120 - 3.100 |
| R-factor | 0.2453 |
| Rwork | 0.244 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.093 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.120 | 3.150 |
| High resolution limit [Å] | 3.099 | 3.100 |
| Rmerge | 0.200 | 0.516 |
| Number of reflections | 21563 | 1029 |
| <I/σ(I)> | 17 | 8 |
| Completeness [%] | 100.0 | |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M HEPES (pH=7.0), 0.2 M Li2SO4 and 12% PEG8000 |
