9WM2

Crystal structure of Escherichia coli RecG in complex with a partial replication fork and ADPAlF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-05-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97943
Spacegroup name	P 21 21 21
Unit cell lengths	59.250, 140.452, 176.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.270 - 3.000
R-factor	0.2245
Rwork	0.224
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.010	3.105
High resolution limit [Å]	2.998	2.998
Rmerge	0.125	0.504
Number of reflections	30509	1445
<I/σ(I)>	17.3	5
Completeness [%]	100.0
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M sodium formate, 0.2 M malic acid (pH=7.0) and 17% PEG3350