9WM1
Crystal structure of Escherichia coli RecG in complex with a partial replication fork and ADPBeF3, ternary complex I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-05-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 143.307, 146.312, 264.183 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.920 - 3.100 |
| R-factor | 0.2277 |
| Rwork | 0.227 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.100 | 3.209 |
| High resolution limit [Å] | 3.098 | 3.098 |
| Rmerge | 0.090 | 0.615 |
| Number of reflections | 101414 | 4880 |
| <I/σ(I)> | 12.1 | 2.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M sodium cacodylate (pH=6.7), 8 % gamma-PGA-LM and 0.2 M L-proline |
