9WM0
Escherichia coli RecG in complex with a partial replication fork
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-05-20 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 134.428, 134.428, 153.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.047 - 2.200 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.24920 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.047 | 2.278 |
| High resolution limit [Å] | 2.199 | 2.199 |
| Rmerge | 0.071 | 0.480 |
| Number of reflections | 71727 | 3491 |
| <I/σ(I)> | 23 | 4.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl (pH=7.8), 4% poly-gama-glutamic acid low molecular weight polymer, and 28% PEG400, and 28% PEG400 |
