9WLC
Crystal structure of Mus musculus monoclonal antibody for 1.4 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-08-04 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.787, 61.503, 90.842 |
| Unit cell angles | 90.00, 116.16, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.420 |
| R-factor | 0.20723 |
| Rwork | 0.205 |
| R-free | 0.24597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.570 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.530 | 1.415 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.035 | 0.054 |
| Number of reflections | 115877 | 30509 |
| <I/σ(I)> | 39.5 | 13 |
| Completeness [%] | 99.6 | 72.31 |
| Redundancy | 6.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.05 M Zinc Acetate dihydrate 20% w/v Polyethylene Glycol 3350 |
