9WKS

Crystal structure of hen egg-white lysozyme determined in the commissioning of NanoTerasu MX-ES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NanoTerasu BEAMLINE BL09U MX-ES
Synchrotron site	NanoTerasu
Beamline	BL09U MX-ES
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	78.039, 78.039, 36.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.020 - 1.200
R-factor	0.14243
Rwork	0.141
R-free	0.17008
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.960
Data reduction software	XDS (Jan 19, 2025)
Data scaling software	XDS (Jan 19, 2025)
Phasing software	MOLREP (11.09.02)
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.260
High resolution limit [Å]	1.200	1.200
Rmeas	0.043	0.831
Number of reflections	36264	5711
<I/σ(I)>	28.26	3.11
Completeness [%]	99.8	98.9
Redundancy	12.5	12.42
CC(1/2)	1.000	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	50mM sodium acetate, 1M NaCl