9WJE
N-methyltransferase 1 complexed with SAH and MDM in Chimonanthus praecox
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.999, 46.994, 84.647 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 83.530 - 1.740 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.916 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.530 | 1.840 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 72579 | 10709 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 97.6 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.998 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Ammonium tartrate dibasic pH 7.0, 20% w/v PEG 3350 |
