9WJA
N-methyltransferase 2-like complexed with SAH and PDM in Chimonanthus praecox
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.904, 88.567, 95.766 |
| Unit cell angles | 98.95, 104.04, 97.58 |
Refinement procedure
| Resolution | 25.030 - 2.610 |
| R-factor | 0.2351 |
| Rwork | 0.232 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.588 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.120 | 2.750 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 41122 | 6161 |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 91.3 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.940 | 0.554 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Sodium citrate tribasic dihydrate; 24% w/v PEG 3350; pH8.3 |
