9WJ9
N-methyltransferase 2-like complexed with SAH and MDM in Chimonanthus praecox
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.418, 88.866, 95.611 |
| Unit cell angles | 98.61, 104.63, 97.49 |
Refinement procedure
| Resolution | 22.720 - 2.500 |
| R-factor | 0.1909 |
| Rwork | 0.188 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.566 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.840 | 2.240 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 49475 | 11551 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 96.4 | |
| Redundancy | 2.2 | |
| CC(1/2) | 0.963 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Sodium citrate tribasic dihydrate; 24% w/v PEG 3350; pH8.3 |
