9WJ7
N-methyltransferase 2-like complexed with SAH and NCM in Chimonanthus praecox
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.378, 47.451, 84.888 |
| Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.900 |
| R-factor | 0.1782 |
| Rwork | 0.177 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.777 |
| Data reduction software | PROCOR |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 57987 | 3874 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.996 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Sodium citrate tribasic dihydrate; 24% w/v PEG 3350; pH8.3 |
