9WIA

Crystal structure of Helicoverpa armigera oral secretion R-like protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	62.866, 62.866, 160.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 1.800
R-factor	0.2161
Rwork	0.215
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.826
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	56897	2641
<I/σ(I)>	22.9	1.4
Completeness [%]	99.5
Redundancy	12.3
CC(1/2)	0.980	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	0.08 M Tris hydrochloride, 1.6 M Ammonium phosphate monobasic, 20% Glycerol