9WES
Crystal Structure of AflRab7 from Aspergillus flavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.111, 62.111, 110.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.650 - 1.910 |
| R-factor | 0.2099 |
| Rwork | 0.209 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.151 | 0.685 |
| Number of reflections | 19919 | 1904 |
| <I/σ(I)> | 48.08 | 5.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | (1) 1 M MES, 15% (w/v) PEG 6000, pH 6.0; (2) 0.1 M sodium acetate (pH 4.5), 35% (v/v) |
