9WEN

Crystal structure of the homodimeric porcine interleukin-10 (IL-10)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 2 21 21
Unit cell lengths	38.930, 81.200, 105.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.240 - 2.300
R-factor	0.2125
Rwork	0.211
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.968
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (Phenix1.20.1-4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.200	2.370
High resolution limit [Å]	2.240	2.240
Rmerge	0.085	0.735
Number of reflections	16740	2369
<I/σ(I)>	13.6
Completeness [%]	99.9
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M sodium fluoride, 20% w/v polyethylene glycol (PEG) 3,350