9WDL

Crystal Structure of Anti-CRISPR protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	25.340, 37.170, 40.700
Unit cell angles	114.10, 90.10, 104.70

Refinement procedure

Resolution	21.530 - 1.730
R-factor	0.2147
Rwork	0.213
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.790
High resolution limit [Å]	1.730	5.000	1.730
Rmerge	0.082	0.050	0.680
Rmeas	0.097	0.060	0.797
Number of reflections	13091	526	1285
<I/σ(I)>	8.67
Completeness [%]	95.8
Redundancy	3.5
CC(1/2)	0.995	0.994	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M HEPES-NaOH, pH 7.0, 0.2 M Sodium Chloride, 25 % (w/v) PEG3350