9WDJ
Crystal structure of PI5P4K beta apo form using low energy X-ray
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2025-03-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.9000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.924, 184.032, 105.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.690 - 3.150 |
| R-factor | 0.2131 |
| Rwork | 0.210 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bo1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.993 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 3.370 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rpim | 0.032 | 0.416 |
| Number of reflections | 18396 | 3278 |
| <I/σ(I)> | 18.9 | 2.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 8%(w/v) PEG4000, 100 mM sodium citrate (pH 5.5), 100 mM magnesium acetate, 100mM lithium acetate |
