9WDG
TenA family transcriptional regulator-LysR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-20 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 75.193, 75.634, 102.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.380 - 1.670 |
| R-factor | 0.18569 |
| Rwork | 0.185 |
| R-free | 0.20268 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.860 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.620 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 34027 | 4513 |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 98.6 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.999 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 1.0 M sodium acetate trihydrate, 0.1 M sodium HEPES, pH 7.5 |
