9WBP

Crystal Structure of Pseudomonas aeruginosa SuhB in complex with Adenosine 2'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2025-05-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97893
Spacegroup name	P 21 21 21
Unit cell lengths	65.696, 90.051, 113.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.070 - 2.000
R-factor	0.2058
Rwork	0.203
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.238
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.070	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.402
Rmeas	0.074	0.473
Rpim	0.028	0.241
Number of reflections	45736	6170
<I/σ(I)>	14.6	2.3
Completeness [%]	98.9	93
Redundancy	6.5	3.6
CC(1/2)	0.999	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Sodium acetate trihydrate and PEG3350