9WBM
O-methyltransferase CcOMT8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.990, 78.040, 90.620 |
| Unit cell angles | 86.22, 89.57, 84.28 |
Refinement procedure
| Resolution | 49.210 - 2.580 |
| R-factor | 0.27149 |
| Rwork | 0.269 |
| R-free | 0.30957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.210 | 2.670 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Number of reflections | 47760 | 4596 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 94.5 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.987 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 100mM Tris base HCl pH 8.5, 500mM Sodium chloride, 8% PEG 8000, 30% MPD |
