9WAU

Crystal Structure of Chromobacterium violaceum Virulence Protein CteX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	CCD
Collection date	2023-01-08
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	56.139, 63.041, 65.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.050 - 1.500
R-factor	0.1875
Rwork	0.185
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.443
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.050	1.558
High resolution limit [Å]	1.500	1.504
Rmerge		0.069
Number of reflections	37433	30798
<I/σ(I)>	15.86
Completeness [%]	97.2
Redundancy	7
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	1 M ammonium sulfate, 0.1 M HEPES (pH 7.0), 0.8% (w/v) PEG 8000, and 15% (v/v) glycerol