9WAQ

Crystal structure of the effector-immunity VP14460-VP14465 in Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	200
Detector technology	CCD
Collection date	2024-05-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	53.836, 58.476, 80.131
Unit cell angles	89.90, 89.84, 62.92

Refinement procedure

Resolution	31.760 - 1.970
R-factor	0.2113
Rwork	0.209
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m38
RMSD bond length	0.014
RMSD bond angle	1.784
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHASES
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.760	2.467
High resolution limit [Å]	1.970	1.970
Number of reflections	57967	5555
<I/σ(I)>	1.71
Completeness [%]	99.6
Redundancy	1.71
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M potassium chloride, 20% w/v polyethylene glycol 3350