9WA8

Crystal structure of AcrIIA19 from Staphylococcus pseudintermedius

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2023-03-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	63.410, 98.980, 196.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.950 - 1.980
R-factor	0.2405
Rwork	0.239
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.821
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.950	2.051
High resolution limit [Å]	1.980	1.980
Number of reflections	42893	4270
<I/σ(I)>	24.29
Completeness [%]	97.9
Redundancy	13.2
CC(1/2)	1.000	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M HEPES pH 8.1, 1.8M Ammonium Sulfate