9W7W
Crystal Structure of NFIB Bound to a Palindromic DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.952, 125.551, 68.004 |
| Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
| Resolution | 31.390 - 2.810 |
| R-factor | 0.2499 |
| Rwork | 0.246 |
| R-free | 0.29270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9w7s |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.491 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.164 | |
| Rpim | 0.068 | 0.793 |
| Number of reflections | 13562 | 260 |
| <I/σ(I)> | 30.4 | |
| Completeness [%] | 62.4 | |
| Redundancy | 5.6 | 3.9 |
| CC(1/2) | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Potassium Sodium Tartrate and 20% (w/v) PEG 3350 |
