9W7S
Crystal Structure of NFIB Bound to TGGCA-Containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100.15 |
| Detector technology | PIXEL |
| Collection date | 2024-11-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.873, 54.183, 58.721 |
| Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
| Resolution | 39.460 - 2.300 |
| R-factor | 0.2219 |
| Rwork | 0.217 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9jh2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.557 |
| Data reduction software | autoPX |
| Data scaling software | autoPX |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.230 | 0.916 |
| Rmeas | 0.251 | 0.999 |
| Rpim | 0.100 | 0.393 |
| Number of reflections | 11016 | 1586 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 98.7 | 97.8 |
| Redundancy | 6.36 | 6.27 |
| CC(1/2) | 0.983 | 0.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.04 M Potassium Phosphate monobasic, 16% (w/v) PEG 8000, and 20% (v/v) Glycerol |
