9W7G

Crystal structure of African swine fever virus methyltransferase EP424R in complex with sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	80.211, 57.777, 101.912
Unit cell angles	90.00, 112.05, 90.00

Refinement procedure

Resolution	19.540 - 2.880
R-factor	0.1796
Rwork	0.177
R-free	0.22870
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.730
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.460	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	43288	792
<I/σ(I)>	8.2
Completeness [%]	63.5
Redundancy	3.9
CC(1/2)	0.990	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15%(w/v) PEG 3350, 200 mM Ammonium citrate dibasic