9W7F
Crystal structure of African swine fever virus methyltransferase EP424R in complex with S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.596, 57.723, 101.429 |
| Unit cell angles | 90.00, 111.44, 90.00 |
Refinement procedure
| Resolution | 50.070 - 2.590 |
| R-factor | 0.2064 |
| Rwork | 0.203 |
| R-free | 0.27590 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.070 | 2.660 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 26698 | 1950 |
| <I/σ(I)> | 0.87 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.1 | 4.9 |
| CC(1/2) | 0.925 | 0.358 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20%(w/v) PEG 3350, 200 mM Ammonium citrate dibasic |
