9W6Y
Crystal structure of L-galactose dehydrogenase from Luteolibacter sp. strain LG18 in complex with L-galactose and NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2021-08-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.297, 90.510, 96.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.290 - 1.500 |
| Rwork | 0.167 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.444 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 1.380 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 141062 | 3379 |
| <I/σ(I)> | 36.8 | |
| Completeness [%] | 93.1 | |
| Redundancy | 12.1 | |
| CC(1/2) | 0.999 | 0.942 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30%(w/v) polyethylene glycol 8000 200 mM ammonium sulfate |
