9W6X

Crystal Structure of YtkW in Complex with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	I 41 2 2
Unit cell lengths	63.799, 63.799, 238.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.200 - 2.380
R-factor	0.1996
Rwork	0.197
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.638
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.630	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	10418	485
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	12.4
CC(1/2)	0.995	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	1 M succinic acid-sodium hydroxide(pH 7.0)