9W6C

Crystal structure of Cas5IC from Moraxella bovoculi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	125
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	P 1 21 1
Unit cell lengths	73.030, 92.980, 83.820
Unit cell angles	90.00, 96.99, 90.00

Refinement procedure

Resolution	29.490 - 3.330
R-factor	0.2247
Rwork	0.222
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.594
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.490	3.449
High resolution limit [Å]	3.330	3.330
Number of reflections	16138	1600
<I/σ(I)>	8.83
Completeness [%]	98.2
Redundancy	6.4
CC(1/2)	0.988	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10% (w/v) PEG 8000, 0.1M HEPES pH 7.5, 8% (v/v) Etylene glycol