9W5Z

Structure of heme transport protein Shr-Linker-NEAT1 from Streptococcus pyogenes in complex with heme.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	42.585, 113.246, 44.281
Unit cell angles	90.00, 96.44, 90.00

Refinement procedure

Resolution	42.320 - 2.350
R-factor	0.21821
Rwork	0.216
R-free	0.26417
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.298
Data reduction software	XDS (20241002)
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.320	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.137	0.697
Rpim	0.055	0.277
Number of reflections	17354	1669
<I/σ(I)>	11	2.8
Completeness [%]	99.8	99.3
Redundancy	7.1	7.2
CC(1/2)	0.996	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	200 mM NaCl 100 mM BIS-TRIS 25% PEG 3350