9W55

Structure of L-glutamate oxidase E617Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	125.201, 125.201, 170.813
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.780 - 2.430
R-factor	0.1983
Rwork	0.197
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e1m
RMSD bond length	0.002
RMSD bond angle	0.520
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.600	2.520
High resolution limit [Å]	2.430	2.430
Number of reflections	30455	3144
<I/σ(I)>	10.8	3.1
Completeness [%]	99.9	100
Redundancy	5.3	5.6
CC(1/2)	0.996	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris-HCl (pH 7.0), 2.5 M NaCl, and 0.2 M MgCl2