9W4U

ASFV E146L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	50.068, 50.137, 50.399
Unit cell angles	77.09, 80.10, 77.78

Refinement procedure

Resolution	25.910 - 2.000
R-factor	0.1863
Rwork	0.184
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.947
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.910	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	29734	2964
<I/σ(I)>	8.9
Completeness [%]	95.0
Redundancy	2.1
CC(1/2)	0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M BIS-TRIS,pH 6.5, 25% PEG 3350