9W47

Structure of protein O-glucosyltransferase 2 with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-02
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	47.130, 63.280, 89.530
Unit cell angles	90.00, 95.96, 90.00

Refinement procedure

Resolution	29.680 - 1.790
R-factor	0.2077
Rwork	0.206
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.580	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.073
Number of reflections	47852	113887
<I/σ(I)>	14.1
Completeness [%]	96.7	96.7
Redundancy	6.3
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293.15	0.2-0.3 M sodium fomate, pH 7.2, and 20-30% w/v polyethylene glycol 3,350