9W2A

Structure of a human heavy chain ferritin nanocage mutant form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-07-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	F 4 3 2
Unit cell lengths	183.267, 183.267, 183.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.400 - 1.550
Rwork	0.193
R-free	0.20110
Structure solution method	SAD
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.400	1.600
High resolution limit [Å]	1.550	1.550
Number of reflections	38657	1984
<I/σ(I)>	30.3
Completeness [%]	95.2
Redundancy	66
CC(1/2)	1.000	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	2 M MgCl2, 0.1 M Bicine, pH 9.0